Fig. 7
Delivery of LV-NR-R80Q-apoB reduces demyelination in MBP-α-syn tg mice. a Vibratome brain sections were stained with luxol fast blue (LFB) and analyzed by bright field microscopy. The panel in the top is a lower magnification view (40X) showing myelin in blue in white matter tracks. The bottom panels are higher magnification (400X) of the corpus callosum of non-tg mice or MBP-α-syn tg mice treated with LV-Control or LV-NR-R80Q-apoB. Scale bars = Overview 250 μm, corpus callosum 50 μm. b Semiquantitative analysis of LFB staining by optical density in the corpus callosum. c Transmission electron microscopy (TEM) images of myelin sheaths in the corpus callosum of non-tg mice or MBP-α-syn tg mice treated with LV-Control or LV-NR-R80Q-apoB. Representative micrographs taken with TEM are shown at low magnification (5,000x) and high magnification (25,000x). Scale bars = Overview 2.5 μm, Corpus callosum 500 nm. d Computer aided image analysis of the number of myelinated axons in corpus callosum. e Tissue blocks containing the corpus callosum and striatum were fractioned by ultracentrifugation and analyzed by immunoblot with antibodies against MBP and CNP. (f) Densitometric analysis of the MBP and CNP signal normalized to the actin signal. * indicates statistical significance (p < 0.05, one way ANOVA, post hoc Dunnett’s) compared to LV-Control treated Non-tg mice. n = 10 mice per group 9–10 m/o at the end of the treatment. # indicates statistical significance (p < 0.05, one way ANOVA, post hoc Tukey-Kramer) compared to LV-Control treated MBP-α-syn tg mice