Fig. 2
Effect of tau and APP on dendritic morphology of principal hippocampal neurons. a Quantitative analysis of dendritic complexity as determined from total path length (top) and number of branching points (bottom). Expression of EGFP-htau induces dendritic simplification in CA1 neurons from APPSDL transgenic mice. b Schematic representation showing the major connectivity in different layers of the hippocampus. Gray boxes indicate sites of Schaffer collateral input in apical dendrites of CA1 neurons. c 3D Sholl analysis of the basal and apical dendritic tree in CA1 neurons. A schematic representation illustrating the procedure to determine dendritic intersections is shown left. Significant changes occur at two segments in the apical dendrite, which are indicated by gray boxes corresponding to the Schaffer collateral input as shown in (b). Statistical evaluation was performed using two-tailed, unpaired Student’s t test for comparison of the two genotypes in (a) and (c). Values are shown as mean and s.e.m. *, p < 0.05, **, p < 0.01, ***, p < 0.001. See Table 1 for details on the statistics