Table 1 Dendritic simplification in an ex vivo model of Alzheimer’s disease. Summary representation of the effect of the expressed construct, the genotype of the mouse and pharmacological treatments on total path length (A) and number of branching points (B). Statistical evaluation was performed using two-tailed, unpaired Student’s t test for comparison of the two genotypes under control conditions (untreated), and one way ANOVA with post hoc Fisher’s LSD test for multiple comparisons of drug treatments or different tau constructs. P values, which represent statistical significance (p≤0.05) are indicated in bold, except when the alpha level of ANOVA was above 0.05
A. Total path length | |||||||||
Construct | Condition | Region | Genotype | Mice (n) | Cells (n) | Mean ± s.e.m (based on cell number) in mm | Student’s test | ||
p values (B6 vs. APP) | |||||||||
EGFP | untreated | CA1 | B6 | 5 | 9 | 3.113±0.465 | p = 0.775 | ||
EGFP | untreated | CA1 | APPSDL | 5 | 5 | 3.364±0.707 | |||
EGFP | untreated | CA3 | B6 | 7 | 8 | 4.490±0.292 | p = 0.401 | ||
EGFP | untreated | CA3 | APPSDL | 5 | 7 | 4.187±0.202 | |||
EGFP | untreated | DG | B6 | 6 | 6 | 1.223±0.232 | p = 0.275 | ||
EGFP | untreated | DG | APPSDL | 5 | 7 | 0.911±0.161 | |||
EGFP-htau | untreated | CA1 | B6 | 4 | 7 | 3.996±0.237 | p = 0.0003 | ||
EGFP-htau | untreated | CA1 | APPSDL | 6 | 8 | 2.491±0.163 | |||
EGFP-htau | untreated | CA3 | B6 | 5 | 6 | 4.344±0.423 | p = 0.428 | ||
EGFP-htau | untreated | CA3 | APPSDL | 5 | 6 | 3.920±0.283 | |||
EGFP-htau | untreated | DG | B6 | 5 | 10 | 0.813±0.071 | p = 0.888 | ||
EGFP-htau | untreated | DG | APPSDL | 7 | 17 | 0.826±0.051 | |||
Construct | Condition | Region | Genotype | Mice (n) | Cells (n) | Mean ± s.e.m (based on cell number) in mm | One way ANOVA (together with EGFP-htau untreated, both genotypes, same region) | p values (B6 vs. APP) | p values ( vs. untreated EGFP-htau same region and same genotype) |
EGFP-htau | DAPT | CA1 | B6 | 4 | 6 | 3.691±0.154 | F (3,24) = 14.05 ; p < 0.0001 | p = 0.0440 | p = 0.2657 |
EGFP-htau | DAPT | CA1 | APPSDL | 4 | 7 | 3.122±0.154 | p = 0.0182 | ||
EGFP-htau | DAPT | CA3 | B6 | 5 | 6 | 3.122±0.258 | F (3,22) = 3.634 ; p = 0.0287 | p = 0.8981 | p = 0.0133 |
EGFP-htau | DAPT | CA3 | APPSDL | 4 | 8 | 3.177±0.249 | p = 0.0940 | ||
EGFP-htau | CPP | CA1 | B6 | 5 | 7 | 3.671±0.289 | F (3,25) = 7.781 ; p = 0.0008 | p = 0.2469 | p = 0.3517 |
EGFP-htau | CPP | CA1 | APPSDL | 6 | 7 | 3.265±0.263 | p = 0.0279 | ||
EGFP-htau | CPP | CA3 | B6 | 6 | 6 | 3.714±0.293 | F (3,23) = 0.5765 ; p = 0.6363 | p = 0.8658 | p = 0.2483 |
EGFP-htau | CPP | CA3 | APPSDL | 7 | 9 | 3.797±0.357 | p = 0.8023 | ||
EGFP-htau | EpoD | CA1 | B6 | 7 | 8 | 3.038±0.288 | F (3,27) = 7.040 ; p = 0.0012 | p = 0.5142 | p = 0.0092 |
EGFP-htau | EpoD | CA1 | APPSDL | 8 | 8 | 2.820±0.241 | p = 0.3273 | ||
EGFP-htau | EpoD | CA3 | B6 | 7 | 8 | 3.603±0.186 | F (3,23) = 5.757 ; p = 0.0043 | p = 0.0416 | p = 0.0527 |
EGFP-htau | EpoD | CA3 | APPSDL | 7 | 7 | 2.853±0.126 | p = 0.0089 | ||
EGFP-Ala htau | untreated | CA1 | B6 | 5 | 7 | 2.797±0.268 | F (3,24) = 8.839 ; p = 0.0004 | p = 0.1005 | p = 0.0011 |
EGFP-Ala htau | untreated | CA1 | APPSDL | 4 | 6 | 3.370±0.249 | p = 0.0125 | ||
EGFP-Ala htau | untreated | CA3 | B6 | 5 | 6 | 2.912±0.224 | F (3,20) = 4.276 ; p = 0.0174 | p = 0.8829 | p = 0.0076 |
EGFP-Ala htau | untreated | CA3 | APPSDL | 4 | 6 | 2.984±0.396 | p = 0.0667 | ||
EGFP-PHP htau | untreated | CA1 | B6 | 4 | 7 | 3.342±0.298 | F (3,24) = 5.250 ; p = 0.0063 | p = 0.6532 | p = 0.1107 |
EGFP-PHP htau | untreated | CA1 | APPSDL | 4 | 6 | 3.155±0.429 | p = 0.1091 | ||
EGFP-PHP htau | untreated | CA3 | B6 | 5 | 6 | 3.578±0.450 | F (3,20) = 2.724 ; p = 0.0714 | p = 0.1941 | p = 0.1722 |
EGFP-PHP htau | untreated | CA3 | APPSDL | 4 | 6 | 2.851±0.352 | p = 0.0621 | ||
Construct | Condition | Region | Genotype | Mice (n) | Cells (n) | Mean ± s.e.m (based on cell number) in mm | One way ANOVA (together with EGFP untreated, both genotypes, same region) | p values (B6 vs. APP) | p values ( vs. untreated EGFP same region and genotype) |
EGFP | CPP | CA1 | B6 | 6 | 7 | 3.597±0.207 | F (3,23) = 0.4707 ; p = 0.7056 | p = 0.7702 | p = 0.4144 |
EGFP | CPP | CA1 | APPSDL | 6 | 6 | 3.787±0.334 | p = 0.5514 | ||
EGFP | CPP | CA3 | B6 | 5 | 5 | 3.603±0.244 | F (3,22) = 2.806 ; p = 0.0634 | p = 0.7762 | p = 0.0473 |
EGFP | CPP | CA3 | APPSDL | 5 | 6 | 3.474±0.382 | p = 0.0974 | ||
EGFP | EpoD | CA1 | B6 | 6 | 6 | 3.110±0.307 | F (3,23) = 0.2951 ; p = 0.8285 | p = 0.4532 | p = 0.9960 |
EGFP | EpoD | CA1 | APPSDL | 7 | 7 | 3.591±0.168 | p = 0.7353 | ||
EGFP | EpoD | CA3 | B6 | 6 | 6 | 2.995±0.268 | F (3,23) = 5.693 ; p = 0.0046 | p = 0.0438 | p = 0.0006 |
EGFP | EpoD | CA3 | APPSDL | 6 | 6 | 3.851±0.287 | p = 0.3939 | ||
B. Number of branching points | |||||||||
Construct | Condition | Region | Genotype | Mice (n) | Cells (n) | Mean ± s.e.m (based on cell number) | Student’s t test | ||
p values (B6 vs. APP) | |||||||||
EGFP | untreated | CA1 | B6 | 5 | 9 | 31±4.11 | p = 0.571 | ||
EGFP | untreated | CA1 | APPSDL | 5 | 5 | 36±7.22 | |||
EGFP | untreated | CA3 | B6 | 7 | 8 | 39±3.83 | p = 0.203 | ||
EGFP | untreated | CA3 | APPSDL | 5 | 7 | 33±1.90 | |||
EGFP | untreated | DG | B6 | 6 | 6 | 11±2.68 | p = 0.261 | ||
EGFP | untreated | DG | APPSDL | 5 | 7 | 7±0.57 | |||
EGFP-htau | untreated | CA1 | B6 | 4 | 7 | 36±3.58 | p = 0.016 | ||
EGFP-htau | untreated | CA1 | APPSDL | 6 | 8 | 23±3.05 | |||
EGFP-htau | untreated | CA3 | B6 | 5 | 6 | 40±3.24 | p = 0.185 | ||
EGFP-htau | untreated | CA3 | APPSDL | 5 | 6 | 34±2.24 | |||
EGFP-htau | untreated | DG | B6 | 5 | 10 | 8±4.49 | p = 0.933 | ||
EGFP-htau | untreated | DG | APPSDL | 7 | 17 | 8±2.73 | |||
Construct | Condition | Region | Genotype | Mice (n) | Cells (n) | Mean ± s.e.m (based on cell number) | One way ANOVA (together with EGFP-htau untreated, both genotypes, same region) | p values (B6 vs. APP) | p values ( vs. untreated EGFP-htau same region and genotype) |
EGFP-htau | DAPT | CA1 | B6 | 4 | 6 | 36±2.11 | F (3,24) = 4.920 ; p = 0.0084 | p = 0.2443 | P > 0.9999 |
EGFP-htau | DAPT | CA1 | APPSDL | 4 | 7 | 31±2.00 | p = 0.0511 | ||
EGFP-htau | DAPT | CA3 | B6 | 5 | 6 | 30±1.95 | F (3,22) = 8.519 ; p = 0.0006 | p = 0.1167 | p = 0.0058 |
EGFP-htau | DAPT | CA3 | APPSDL | 4 | 8 | 25±1.51 | p = 0.0076 | ||
EGFP-htau | CPP | CA1 | B6 | 5 | 7 | 32±4.34 | F (3,25) = 2.434 ; p = 0.0885 | p = 0.4497 | p = 0.4497 |
EGFP-htau | CPP | CA1 | APPSDL | 6 | 7 | 28±3.53 | p = 0.3310 | ||
EGFP-htau | CPP | CA3 | B6 | 6 | 6 | 32±2.77 | F (3,23) = 5.381 ; p = 0.0059 | p = 0.1041 | p = 0.0509 |
EGFP-htau | CPP | CA3 | APPSDL | 7 | 9 | 26±2.19 | p = 0.0339 | ||
EGFP-htau | EpoD | CA1 | B6 | 7 | 8 | 27±1.83 | F (3,27) = 4.824 ; p = 0.0081 | p = 0.5753 | p = 0.0203 |
EGFP-htau | EpoD | CA1 | APPSDL | 8 | 8 | 25±1.31 | p = 0.5753 | ||
EGFP-htau | EpoD | CA3 | B6 | 7 | 8 | 29±2.86 | F (3,23) = 4.362 ; p = 0.0143 | p = 0.4458 | p = 0.0120 |
EGFP-htau | EpoD | CA3 | APPSDL | 7 | 7 | 26±2.93 | p = 0.0668 | ||
EGFP-Ala htau | untreated | CA1 | B6 | 5 | 7 | 26±3.41 | F (3,24) = 3.655 ; p = 0.0266 | p = 0.1135 | p = 0.0430 |
EGFP-Ala htau | untreated | CA1 | APPSDL | 4 | 6 | 34±3.12 | p = 0.0288 | ||
EGFP-Ala htau | untreated | CA3 | B6 | 5 | 6 | 25±3.06 | F (3,20) = 4.572 ; p = 0.0135 | p = 0.5032 | p = 0.0028 |
EGFP-Ala htau | untreated | CA3 | APPSDL | 4 | 6 | 28±3.72 | p = 0.1878 | ||
EGFP-PHP htau | untreated | CA1 | B6 | 4 | 7 | 32±3.28 | F (3,24) = 3.736 ; p = 0.0246 | p = 0.1469 | p = 0.3815 |
EGFP-PHP htau | untreated | CA1 | APPSDL | 4 | 6 | 25±2.45 | p = 0.6630 | ||
EGFP-PHP htau | untreated | CA3 | B6 | 5 | 6 | 38±6.57 | F (3,20) = 0.6744 ; p = 0.5778 | p = 0.3253 | p = 0.7762 |
EGFP-PHP htau | untreated | CA3 | APPSDL | 4 | 6 | 31±6.14 | p = 0.6702 | ||
Construct | Condition | Region | Genotype | Mice (n) | Cells (n) | Mean ± s.e.m (based on cell number) | One way ANOVA (together with EGFP untreated, both genotypes, same region) | p values (B6 vs. APP) | p values ( vs. untreated EGFP same region and genotype) |
EGFP | CPP | CA1 | B6 | 6 | 7 | 34±3.15 | F (3,23) = 0.3279 ; p = 0.8052 | p = 0.7518 | p = 0.6011 |
EGFP | CPP | CA1 | APPSDL | 6 | 6 | 36±2.74 | p > 0.9999 | ||
EGFP | CPP | CA3 | B6 | 5 | 5 | 33±3.24 | F (3,22) = 2.872 ; p = 0.0594 | p = 0.1730 | p = 0.2132 |
EGFP | CPP | CA3 | APPSDL | 5 | 6 | 26±3.16 | p = 0.1396 | ||
EGFP | EpoD | CA1 | B6 | 6 | 6 | 25±2.16 | F (3,23) = 1.069 ; p = 0.3814 | p = 0.3033 | p = 0.2778 |
EGFP | EpoD | CA1 | APPSDL | 7 | 7 | 31±0.57 | p = 0.4130 | ||
EGFP | EpoD | CA3 | B6 | 6 | 6 | 23±3.24 | F (3,23) = 4.970 ; p = 0.0084 | p = 0.0141 | p = 0.0010 |
EGFP | EpoD | CA3 | APPSDL | 6 | 6 | 35±1.99 | p = 0.6501 | ||