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Table 1 Dendritic simplification in an ex vivo model of Alzheimer’s disease. Summary representation of the effect of the expressed construct, the genotype of the mouse and pharmacological treatments on total path length (A) and number of branching points (B). Statistical evaluation was performed using two-tailed, unpaired Student’s t test for comparison of the two genotypes under control conditions (untreated), and one way ANOVA with post hoc Fisher’s LSD test for multiple comparisons of drug treatments or different tau constructs. P values, which represent statistical significance (p≤0.05) are indicated in bold, except when the alpha level of ANOVA was above 0.05

From: Region-specific dendritic simplification induced by Aβ, mediated by tau via dysregulation of microtubule dynamics: a mechanistic distinct event from other neurodegenerative processes

A. Total path length
Construct Condition Region Genotype Mice (n) Cells (n) Mean ± s.e.m (based on cell number) in mm Student’s test   
p values (B6 vs. APP)
EGFP untreated CA1 B6 5 9 3.113±0.465 p = 0.775
EGFP untreated CA1 APPSDL 5 5 3.364±0.707
EGFP untreated CA3 B6 7 8 4.490±0.292 p = 0.401
EGFP untreated CA3 APPSDL 5 7 4.187±0.202
EGFP untreated DG B6 6 6 1.223±0.232 p = 0.275
EGFP untreated DG APPSDL 5 7 0.911±0.161
EGFP-htau untreated CA1 B6 4 7 3.996±0.237 p = 0.0003
EGFP-htau untreated CA1 APPSDL 6 8 2.491±0.163
EGFP-htau untreated CA3 B6 5 6 4.344±0.423 p = 0.428
EGFP-htau untreated CA3 APPSDL 5 6 3.920±0.283
EGFP-htau untreated DG B6 5 10 0.813±0.071 p = 0.888
EGFP-htau untreated DG APPSDL 7 17 0.826±0.051
Construct Condition Region Genotype Mice (n) Cells (n) Mean ± s.e.m (based on cell number) in mm One way ANOVA (together with EGFP-htau untreated, both genotypes, same region) p values (B6 vs. APP) p values ( vs. untreated EGFP-htau same region and same genotype)
EGFP-htau DAPT CA1 B6 4 6 3.691±0.154 F (3,24) = 14.05 ; p < 0.0001 p = 0.0440 p = 0.2657
EGFP-htau DAPT CA1 APPSDL 4 7 3.122±0.154 p = 0.0182
EGFP-htau DAPT CA3 B6 5 6 3.122±0.258 F (3,22) = 3.634 ; p = 0.0287 p = 0.8981 p = 0.0133
EGFP-htau DAPT CA3 APPSDL 4 8 3.177±0.249 p = 0.0940
EGFP-htau CPP CA1 B6 5 7 3.671±0.289 F (3,25) = 7.781 ; p = 0.0008 p = 0.2469 p = 0.3517
EGFP-htau CPP CA1 APPSDL 6 7 3.265±0.263 p = 0.0279
EGFP-htau CPP CA3 B6 6 6 3.714±0.293 F (3,23) = 0.5765 ; p = 0.6363 p = 0.8658 p = 0.2483
EGFP-htau CPP CA3 APPSDL 7 9 3.797±0.357 p = 0.8023
EGFP-htau EpoD CA1 B6 7 8 3.038±0.288 F (3,27) = 7.040 ; p = 0.0012 p = 0.5142 p = 0.0092
EGFP-htau EpoD CA1 APPSDL 8 8 2.820±0.241 p = 0.3273
EGFP-htau EpoD CA3 B6 7 8 3.603±0.186 F (3,23) = 5.757 ; p = 0.0043 p = 0.0416 p = 0.0527
EGFP-htau EpoD CA3 APPSDL 7 7 2.853±0.126 p = 0.0089
EGFP-Ala htau untreated CA1 B6 5 7 2.797±0.268 F (3,24) = 8.839 ; p = 0.0004 p = 0.1005 p = 0.0011
EGFP-Ala htau untreated CA1 APPSDL 4 6 3.370±0.249 p = 0.0125
EGFP-Ala htau untreated CA3 B6 5 6 2.912±0.224 F (3,20) = 4.276 ; p = 0.0174 p = 0.8829 p = 0.0076
EGFP-Ala htau untreated CA3 APPSDL 4 6 2.984±0.396 p = 0.0667
EGFP-PHP htau untreated CA1 B6 4 7 3.342±0.298 F (3,24) = 5.250 ; p = 0.0063 p = 0.6532 p = 0.1107
EGFP-PHP htau untreated CA1 APPSDL 4 6 3.155±0.429 p = 0.1091
EGFP-PHP htau untreated CA3 B6 5 6 3.578±0.450 F (3,20) = 2.724 ; p = 0.0714 p = 0.1941 p = 0.1722
EGFP-PHP htau untreated CA3 APPSDL 4 6 2.851±0.352 p = 0.0621
Construct Condition Region Genotype Mice (n) Cells (n) Mean ± s.e.m (based on cell number) in mm One way ANOVA (together with EGFP untreated, both genotypes, same region) p values (B6 vs. APP) p values ( vs. untreated EGFP same region and genotype)
EGFP CPP CA1 B6 6 7 3.597±0.207 F (3,23) = 0.4707 ; p = 0.7056 p = 0.7702 p = 0.4144
EGFP CPP CA1 APPSDL 6 6 3.787±0.334 p = 0.5514
EGFP CPP CA3 B6 5 5 3.603±0.244 F (3,22) = 2.806 ; p = 0.0634 p = 0.7762 p = 0.0473
EGFP CPP CA3 APPSDL 5 6 3.474±0.382 p = 0.0974
EGFP EpoD CA1 B6 6 6 3.110±0.307 F (3,23) = 0.2951 ; p = 0.8285 p = 0.4532 p = 0.9960
EGFP EpoD CA1 APPSDL 7 7 3.591±0.168 p = 0.7353
EGFP EpoD CA3 B6 6 6 2.995±0.268 F (3,23) = 5.693 ; p = 0.0046 p = 0.0438 p = 0.0006
EGFP EpoD CA3 APPSDL 6 6 3.851±0.287 p = 0.3939
B. Number of branching points
Construct Condition Region Genotype Mice (n) Cells (n) Mean ± s.e.m (based on cell number) Student’s t test   
p values (B6 vs. APP)
EGFP untreated CA1 B6 5 9 31±4.11 p = 0.571
EGFP untreated CA1 APPSDL 5 5 36±7.22
EGFP untreated CA3 B6 7 8 39±3.83 p = 0.203
EGFP untreated CA3 APPSDL 5 7 33±1.90
EGFP untreated DG B6 6 6 11±2.68 p = 0.261
EGFP untreated DG APPSDL 5 7 7±0.57
EGFP-htau untreated CA1 B6 4 7 36±3.58 p = 0.016
EGFP-htau untreated CA1 APPSDL 6 8 23±3.05
EGFP-htau untreated CA3 B6 5 6 40±3.24 p = 0.185
EGFP-htau untreated CA3 APPSDL 5 6 34±2.24
EGFP-htau untreated DG B6 5 10 8±4.49 p = 0.933
EGFP-htau untreated DG APPSDL 7 17 8±2.73
Construct Condition Region Genotype Mice (n) Cells (n) Mean ± s.e.m (based on cell number) One way ANOVA (together with EGFP-htau untreated, both genotypes, same region) p values (B6 vs. APP) p values ( vs. untreated EGFP-htau same region and genotype)
EGFP-htau DAPT CA1 B6 4 6 36±2.11 F (3,24) = 4.920 ; p = 0.0084 p = 0.2443 P > 0.9999
EGFP-htau DAPT CA1 APPSDL 4 7 31±2.00 p = 0.0511
EGFP-htau DAPT CA3 B6 5 6 30±1.95 F (3,22) = 8.519 ; p = 0.0006 p = 0.1167 p = 0.0058
EGFP-htau DAPT CA3 APPSDL 4 8 25±1.51 p = 0.0076
EGFP-htau CPP CA1 B6 5 7 32±4.34 F (3,25) = 2.434 ; p = 0.0885 p = 0.4497 p = 0.4497
EGFP-htau CPP CA1 APPSDL 6 7 28±3.53 p = 0.3310
EGFP-htau CPP CA3 B6 6 6 32±2.77 F (3,23) = 5.381 ; p = 0.0059 p = 0.1041 p = 0.0509
EGFP-htau CPP CA3 APPSDL 7 9 26±2.19 p = 0.0339
EGFP-htau EpoD CA1 B6 7 8 27±1.83 F (3,27) = 4.824 ; p = 0.0081 p = 0.5753 p = 0.0203
EGFP-htau EpoD CA1 APPSDL 8 8 25±1.31 p = 0.5753
EGFP-htau EpoD CA3 B6 7 8 29±2.86 F (3,23) = 4.362 ; p = 0.0143 p = 0.4458 p = 0.0120
EGFP-htau EpoD CA3 APPSDL 7 7 26±2.93 p = 0.0668
EGFP-Ala htau untreated CA1 B6 5 7 26±3.41 F (3,24) = 3.655 ; p = 0.0266 p = 0.1135 p = 0.0430
EGFP-Ala htau untreated CA1 APPSDL 4 6 34±3.12 p = 0.0288
EGFP-Ala htau untreated CA3 B6 5 6 25±3.06 F (3,20) = 4.572 ; p = 0.0135 p = 0.5032 p = 0.0028
EGFP-Ala htau untreated CA3 APPSDL 4 6 28±3.72 p = 0.1878
EGFP-PHP htau untreated CA1 B6 4 7 32±3.28 F (3,24) = 3.736 ; p = 0.0246 p = 0.1469 p = 0.3815
EGFP-PHP htau untreated CA1 APPSDL 4 6 25±2.45 p = 0.6630
EGFP-PHP htau untreated CA3 B6 5 6 38±6.57 F (3,20) = 0.6744 ; p = 0.5778 p = 0.3253 p = 0.7762
EGFP-PHP htau untreated CA3 APPSDL 4 6 31±6.14 p = 0.6702
Construct Condition Region Genotype Mice (n) Cells (n) Mean ± s.e.m (based on cell number) One way ANOVA (together with EGFP untreated, both genotypes, same region) p values (B6 vs. APP) p values ( vs. untreated EGFP same region and genotype)
EGFP CPP CA1 B6 6 7 34±3.15 F (3,23) = 0.3279 ; p = 0.8052 p = 0.7518 p = 0.6011
EGFP CPP CA1 APPSDL 6 6 36±2.74 p > 0.9999
EGFP CPP CA3 B6 5 5 33±3.24 F (3,22) = 2.872 ; p = 0.0594 p = 0.1730 p = 0.2132
EGFP CPP CA3 APPSDL 5 6 26±3.16 p = 0.1396
EGFP EpoD CA1 B6 6 6 25±2.16 F (3,23) = 1.069 ; p = 0.3814 p = 0.3033 p = 0.2778
EGFP EpoD CA1 APPSDL 7 7 31±0.57 p = 0.4130
EGFP EpoD CA3 B6 6 6 23±3.24 F (3,23) = 4.970 ; p = 0.0084 p = 0.0141 p = 0.0010
EGFP EpoD CA3 APPSDL 6 6 35±1.99 p = 0.6501