Fig. 4
From: SOD1 protein aggregates stimulate macropinocytosis in neurons to facilitate their propagation
Rottlerin and EIPA treatment inhibits the uptake of SOD1 aggregates. a-b Internalization of aggregated wtSOD1 (20 μg/mL) after 30 min at 37 °C, in the absence (no treatment; NT) or presence of a pre-incubation step with either rottlerin (Rot; 3 μM) a PAK-1 and PKC inhibitor, EIPA (100 μM) a Na+/H+ exchange inhibitor, genistein (Gen; 10 μM) a caveolin mediated uptake inhibitor, or chlorpromazine hydrochloride (CPZ; 5 mM) a clathrin mediated endocytosis inhibitor. a The mean fluorescence intensity (MFI) of SOD1 aggregate uptake was determined by flow cytometry. Results shown are means ± SD, n =6, *** P <0.001 (b) Laser scanning confocal micrographs of aggregated wtSOD1 internalized by NSC-34 cells in the presence or absence of a pre-incubation step with rottlerin (Rot), EIPA, genistein (Gen) and chlorpromazine hydrochloride (CPZ)