Fig. 1
Statin treatment induces extracellular secretion of functional insulin-degrading enzyme (IDE) from astrocytes. a & b Increased IDE levels secreted from the primary astrocytes by simvastatin in a concentration-(a) and time-(b) dependent manner. The time points mean the time period of simvastatin treatment. Blots are representative of at least three independent experiments (N = 3 experiments). ** p < 0.01, # p < 0.05, ## p < 0.01 vs. vehicle-treated cells. c Secreted IDE levels by simvastatin with IDE ELISA. d IDE enzymatic activity in the media from simvastatin-treated cells. (e & f) Cell-free Aβ degradation assay. The arrowhead indicates remaining Aβ40 levels. Data were obtained from at least three replicates for each group (N = 3 experiments). rIDE, recombinant IDE protein; Thio, thiorphan; Baci. A, Bacitracin A. ** p < 0.01, *** p < 0.001 vs. vehicle-treated cells; ## p < 0.01 vs. cells treated with simvastatin