Fig. 5
PRAK mediates Aβ-induced autophagy signaling pathway. a SH-SY5Y cells were transfected with plasmids expressing RAGE and then transiently re-transfected with PRAK siRNA or scrambled control siRNA. After 24 h, cells were treated with either DMSO or 2 μM monomeric Aβ for 6 h. b SH-SY5Y cells were transfected with plasmids expressing RAGE and transiently retransfected with PRAK siRNA or scrambled control siRNA. After 24 h, cells were pretreatment with 10 nM Bafilomycin A1 for 1 h and treated with either DMSO or 2 μM monomeric Aβ for 6 h. Western blot analysis was performed with the indicated antibodies. Quantification of protein level was performed by densitometric analysis. Data are mean ± SEM of 5 separate experiments. **P < 0.01. c Cells were co-transfected with RAGE plasmid DNA and PRAK siRNA or scrambled control siRNA. After 24 h, cells were treated with either DMSO or 2 μM monomeric Aβ for 6 h, fixed, and stained for TEM as described in the methods section. EM images indicate PRAK-mediated changes in the number of autophagosomes (arrows). Quantification of autophagosomes is shown in (d). Scale bars represent 1 μm. *P < 0.05