Fig. 2
From: ADAMTS-4 promotes neurodegeneration in a mouse model of amyotrophic lateral sclerosis
Decrease of ADAMTS-4 activity in the lumbar spinal cord of SOD1G93A mice at disease end-stage. a-h Quantitative RT-PCR for ADAMTS-4 (a, e) ADAMTS-1 (b, f) −5 (c, g) and −9 (d, h) expression in the lumbar spinal cord (SC) of WT (blank bar) and SOD1G93A (black bar) male (♂; a, b, c, d) or female (♀; e, f, g, h) mice at presymptomatic (PS), symptomatic (SS) and end (ES) stages. Values plotted are mean ± SEM. Mann–Whitney U-tests: * P < 0.05 compared to corresponding WT mice, $ P < 0.05 compared to SOD1G93A mice at other stages, # P < 0.05 compared to WT mice at other stages, N = 3-4. i Representative photomicrographs of ventral horns in lumbar spinal cord sections from WT and SOD1G93A mice at end-stage stained with ADAMTS-4 (green) and NeuN (neuronal marker, red). Scale bar = 20 μm. j-o Immunoblot for ADAMTS-4 in the lumbar spinal cord of WT and SOD1G93A male (j-l) and female (m-o) mice at PS, SS and ES. The immunoblots revealed ADAMTS-4 mature form (p68), ADAMTS-4 truncated forms (p53, p40) and a 16 kDa fragment. Values plotted are mean ± SEM. Mann–Whitney U-tests: * P < 0.05 compared to corresponding WT mice, N = 4-5. p-q Quantitative RT-PCR for TIMP-3 in the lumbar spinal cord of WT and SOD1G93A male (p) and female (q) mice at PS, SS and ES. Values plotted are mean ± SEM. Mann–Whitney U-tests: * P < 0.05 compared to corresponding WT mice, $ P < 0.05 compared to SOD1G93A mice at other stages, # P < 0.05 compared to WT mice at other stages, N = 3-4. r-s ADAMTS-4 enzymatic activity assay in the lumbar spinal cord of WT and SOD1G93A male (r) and female (s) mice at PS, SS and ES. Values plotted are mean ± SEM. Mann–Whitney U-tests: * P < 0.05 compared to corresponding WT mice, $ P < 0.05 compared to SOD1G93A mice at other stages, # P < 0.05 compared to WT mice at other stages, N = 4