Fig. 2

Overexpressed p53 controls α-syn transcription. SH-SY5Y cells transiently transfected with an empty vector (EV, black bars) or wild-type p53 cDNA (p53, white bars) were assessed for α-syn protein (a and b), promoter transactivation (c) and mRNA levels (d). Bars in a-d represent the means ± SEM of 3-4 independent experiments performed in duplicates or triplicates and are expressed as percentage of control EV cells. Statistical analyses were performed with GraphPad Prism software by using homoscedastic, unpaired Student’s t-test. Significant differences are: **p < 0.01, and ****p < 0.0001