Fig. 6

SP600125 protected against I/R-induced thinning of inner retinal layers. Three doses of SP600125 (5, 15, and 30 mg/kg) or vehicle were administered intraperitoneally 2 h prior to retinal I/R injury followed by once daily dosing. After 28 days, histological changes in retinal thickness, including whole retina (from NFL to ONL), IPL, INL and ONL were analyzed. Data represent mean ± SEM (n = 5) at each time point. The mean thickness of the uninjured, vehicle-treated retinas was set as 100 %. In vehicle-treated group, I/R injury significantly reduced whole retina thickness 23.2 ± 5.7 % compared to non-injured contralateral retinas. In particular, IPL was reduced 38.0 ± 6.7 % and INL reduced 25.1 ± 7.4 %. No significant layer change was observed in ONL. In contrast, no significant layer thickness reduction was observed from SP60025-administered groups at each dose (5, 15 and 30 mg/kg). **: p < 0.01; NS: non-significant between contralateral control and I/R eyes by two-tailed, paired t-test