Fig. 7

SP600125 ameliorated I/R-induced progressive retina thinning. Retinal I/R injured mice were treated with SP600125 (15 mg/kg) or vehicle for 28 days. SD-OCT was used to determine progressive changes in whole retinal layer (from NFL to ONL), and each of IPL, INL, and ONL. a Representative images showing layer changes after 28 days post I/R injury. b Quantitative analyses of progressive changes of thickness of each layer at different time points. Open symbols: I/R eyes; closed symbols: un-injured contralateral eyes. In vehicle-administered groups, whole retina thickness in I/R-injured eyes were progressively decreased at 28 days of injury compared to contralateral non-injured eyes. These changes were mainly observed in inner retinal layers, IPL and INL. In contrast, SP600125 administration inhibited I/R-induced layer reduction. Data are shown as mean and SEM (n = 4). Two-way ANOVA analyses indicate that there were no significant time-dependent changes in all SP600125 groups, but significant differences were detected between the control and I/R groups at certain time points (*: p < 0.05, **: p < 0.01, ***: p < 0.001 by Bonferroni’s post-hoc test). (WRL: whole retina layer, NFL: nerve fiber layer, IPL: inner plexiform layer, INL: inner nuclear layer, ONL: outer nuclear layer)