Fig. 6
Aβ42 protofibril treatment results in formation of enlarged endosomes. Time-lapse experiments demonstrate that Aβ42 protofibril treatment induces the formation of enlarged, dynamic vacuoles (*) in the astrocytes (a). The enlarged vacuoles fuse (white arrow heads) with adjacent vacuoles; forming giant vacuoles with a diameter of 30–50 μm (b). Immunocytochemistry show that the vacuoles express the early endosome marker Rab5 and to a lesser extent the late endosome marker Rab7 (c). Inset in left corner of image c shows higher magnification of relevant structure. Scale bars: a–b = 10 μm, c = 20 μm