Fig. 2
From: Stathmin 1/2-triggered microtubule loss mediates Golgi fragmentation in mutant SOD1 motor neurons
Golgi alterations in mutant SOD1-transfected NSC-34 motor neurons. a. Confocal images (upper panels) showing Golgi fragmentation with the marker MannII-GFP in NSC-34 cells transfected for 4 DIV with mutant SOD1, as compared to the situation in control or wildtype SOD1-transfected cells. Confocal images (lower panels) showing GS28 dispersal after transfection with mutant SOD1, as compared to the situation in control or wildtype SOD1 cells. Scale bar 10 μm. b. Percentage of cells (mean ± sd) with Golgi fragmentation labeled for MannII-GFP. More than 300 cells per condition were analyzed on quadruplicate wells per condition. Statistical significance * p < 0.01 by Mann–Whitney test, as compared to mock and SODwt. Data represent one out of three experiments yielding similar results. c. Percentage of transfected cells with dispersal of GS28. More than 200 cells per condition were analyzed on quadruplicate wells per condition. Statistical significance * p < 0.01 by Mann–Whitney test, as compared to mock and SODwt. d. Western blot analysis of NSC-34 cells demonstrating that GS28 protein levels are increased to 2.7 ± 0.7 and 2.9 ± 0.8 by SOD1G85R and SOD1G93A, respectively, as compared to those in control SODwt. GS15 protein levels are increased to 2.7 ± 0.2 and 3.0 ± 0.3 fold, respectively. The diagrams show densitometric quantification of protein levels relative to SODwt (mean ± sd). Cellular extracts from three independently transfected cultures were blotted and analyzed each in duplicate. Statistical significance * p < 0.01 by Mann Whitney test