Fig. 6

Myeloid-specific ikkβ gene deletion inhibits BBB permeability in the spinal cord with during EAE. a and b The amount of extravasated Evans blue dye was quantified in the brain and lumbar spinal cord, at 15–18 days after immunization (n = 5 per group). Representative pictures (a) and quantitative data (b) are displayed. Blue color in A indicates extravasated Evans blue dye from blood vessels. c-g Representative confocal images of extravasated Evans blue dye in the lumbar spinal cord from each group (n = 5; c-f) and quantified data (g). Red color in the (c-f) indicates extravasated Evans blue dye from blood vessels. h-j The mRNA expression levels of junctional proteins [zona occuluden-1 (h), claudin-3 (i), and claudin-5 (j)] in spinal cords from each group were analyzed by real-time RT-PCR. Data are representative of 3 independent experiments with similar results and the data are expressed as mean fold induction ± SEM. ANOVA test; **p < 0.01 versus supernatant from WT mice; ##p < 0.01 versus supernatant from normal control mice