Fig. 2
Gene expression analysis of Wnts in the hippocampus of EAE mice. a Disease course in passively transferred EAE. Female SJL/J mice received activated encephalitogenic T lymphocytes derived from immunized donors and re-stimulated with encephalitogenic PLP139–151 peptide. Control mice were injected with PBS. Data are shown as mean clinical score ± SEM. Grey columns indicate the time points when mice were sacrificed for analysis: days 6, 20 and 50 after adoptive transfer. b qPCR analysis of Axin2 gene expression in the hippocampus of mice with passively transferred EAE. Data represent mean of fold-changes + SEM of gene expression in EAE mice relative to respective controls set as 1. Expression of Axin2 was normalized to Gapdh. Day 6 (EAE, n = 5; control, n = 4); day 20 (EAE, n = 6; control, n = 8); day 50 (EAE, n = 12; control, n = 11). Two-tailed, unpaired Student’s t-test, *** p < 0.001. c qPCR analysis of selected Wnts genes in the hippocampus at early (day 20) and chronic (day 50) stages of passive EAE. Histogram represents mean + SEM of fold-changes relative to control group set as 1. Gene expression was normalized to Gapdh. Day 20 (EAE, n = 4–6; control, n = 4); day 50 (EAE, n = 8–13; control, n = 7–11). Two-tailed, unpaired Student’s t-test, * p < 0.05, ** p < 0.01 and *** p < 0.001