Fig. 5

Distinct cellular localization of BIN1 and Amphiphysin 1 in the human brain. a Immunohistochemical labeling of adjacent sections of the hippocampus and parahippocampal gyrus using mAb against Amphiphysin 1 or BIN1 (99D) reveals near complementary pattern of labeling. Amphiphysin 1 antibody does not stain the white matter whereas intense BIN1 labeling is observed in the white matter. b Amphiphysin 1 and BIN1 immunolabeling of the cortex (the boxed area in panel A) and a section of the white matter are shown at a higher magnification. Boxed regions in the external granule layer and internal pyramidal layer are also shown at further magnification to visualize the cells that are immunoreactive. Amphiphysin 1 and BIN1 labeling of morphologically distinct neurons and oligodendrocytes, respectively, are quite obvious. c Amphiphysin 1 and BIN1 labeling of the hippocampal area. A higher magnification of the boxed area of CA2 and dentate gyrus is shown at the bottom and on the right, respectively. In all areas, Amphiphysin 1 mAb stains the neuronal soma and the neuropil whereas BIN1 mAb stains smaller cells and profuse branched processes. Very little BIN1 signal is found in neurons. Amphiphysin 1 but not BIN1 localizes to the terminal fields of hippocampal mossy fibers, which are non-myelinated. Amph 1 = Amphiphysin 1; WM = white matter; Alv = alveus; Hipp = hippocampus; Sub = subiculum; EC = entorhinal cortex; CA = caudate nucleus; CP = cerebral peduncle; F = fimbria; LGN = lateral geniculate nucleus; EGL = external granular layer; IPyL = internal pyramidal layer; DG = dentate gyrus; CA2-CA4 = Cornu Ammonis areas 2–4; MF = mossy fibers; PML = polymorphic layer; GCL = granule cell layer; ML = molecular layer; SO = stratum oriens; SP = stratum pyramidale; SR = stratum raidatum