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Fig. 14 | Molecular Neurodegeneration

Fig. 14

From: Affinity of Tau antibodies for solubilized pathological Tau species but not their immunogen or insoluble Tau aggregates predicts in vivo and ex vivo efficacy

Fig. 14

Mechanism of action is influenced by dosing method. PHF (10 μg/ml) was added to JNPL3 neurons, with 4E6 added either at the same time or 24 h later. In additional cultures, 1 μg/ml dansyl cadaverine (DC), an inhibitor of clathrin mediated endocytosis, was also added along with 4E6 to determine whether antibody internalization is necessary for 4E6 to exert its effects. a In the PHF + Ab treatment group, addition of DC did not affect the NeuN corrected tau levels, indicating that the antibody is working extracellularly. b When DC was present in the PHF → Ab condition, total tau/NeuN ratios was shifted beginning at 24 h, and the relative tau levels were significantly higher after 7 days. Hence, under these conditions, the antibody is working intracellularly

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