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Fig. 8 | Molecular Neurodegeneration

Fig. 8

From: Affinity of Tau antibodies for solubilized pathological Tau species but not their immunogen or insoluble Tau aggregates predicts in vivo and ex vivo efficacy

Fig. 8

Low dose PHF (1 μg/ml) increased the intracellular tau/NeuN ratio, which was prevented by 4E6. a, b Immunoblots for samples exposed to PHF alone or with a 4E6 or b control IgG1, probed with a pan tau polyclonal antibody. c Quantitation of total tau levels shows that at 1 μg/ml, PHF promoted significant increases in intracellular tau (95 % above control, p < 0.0001). For 4E6, the PHF + Ab and PHF → Ab were significantly lower than the PHF alone samples (p < 0.0001). Ab → PHF cells were not reduced relative to PHF alone. d IgG did not prevent against significant increases in total tau after exposure to PHF. e As above, the ratio of tau/NeuN was determined, and again this ratio was significantly increased in PHF alone samples relative to control cells (1.6 fold increase, p < 0.0001). Neurons treated with 4E6 in the PHF + Ab and PHF → Ab, but not Ab → PHF groups had significantly lower corrected tau levels compared to the PHF alone groups (p < 0.0001). f When adjusted for NeuN levels tau levels in the PHF + Ab, PHF → Ab, and Ab → PHF control IgG groups were 0.98, 1.4 and 1.3 fold higher than control, and not significantly different from PHF alone

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