Fig. 9From: Affinity of Tau antibodies for solubilized pathological Tau species but not their immunogen or insoluble Tau aggregates predicts in vivo and ex vivo efficacy4E6, but not 6B2, prevented the increase in the phosphorylated tau/NeuN ratio caused by exposure to 10 μg/ml PHF. a–c Samples probed with a polyclonal antibody recognizing tau phosphorylated at Ser199 from cells exposed to PHF alone or PHF in combination with a 4E6, b 6B2 or c control IgG1. d PHF alone samples had significantly reduced phospho-tau levels relative to untreated cells (34 % reduction, p < 0.05). For 4E6, both the PHF + Ab and PHF → Ab treatment groups had significantly higher phospho-tau levels than the PHF alone group (p < 0.001 and 0.05 respectively). e In samples treated with a combination of PHF and 6B2, none of the treatment groups were significantly different from PHF alone. f As with 6B2, uncorrected IgG1 samples showed no significant difference relative to PHF alone. g Correcting for NeuN levels to take PHF toxicity into account, PHF alone samples had higher ratio of P-Ser199/NeuN, compared to untreated controls (a 4.1 fold increase, p < 0.0001). Phospho- tau levels in the 4E6 PHF + Ab and PHF → Ab groups were significantly lower than the PHF alone samples (91 and 78 % of untreated controls, p < 0.0001). h When corrected for NeuN levels, 6B2 treated cells were not significantly different from PHF alone samples. i When NeuN levels were considered, IgG1 samples were also not significantly different from PHF aloneBack to article page