Fig. 3

Increase in the stable PS1 complexes in AD and MCI CSF. a Representative blot and (b) densitometric quantification of the accumulative immunoreactivity from the sum of stable higher molecular mass PS1 complexes (100 + 150 kDa) in CSF samples from 13 sAD, 12 MCI and 17 age-matched eNC subjects. A quotient calculated as the sum of (100 + 150 kDa) immunoreactivity relative to the 50 kDa immunoreactivity: (100 + 150 kDa/50 kDa) is also shown. c Six AD and MCI individuals, and 8 eNC subjects were fractionated on 5–20 % sucrose density gradients, and probed with the PS1 antibody under denaturing conditions. The internal markers were β-galactosidase (G), catalase (C) and alkaline phosphatase (P), as in Fig. 3. d The values for the “stability” quotient reflecting the highly stable complexes (100 + 150 kDa immunoreactive bands sedimenting in fractions 2–7) relative to the unstable complexes (50 kDa immunoreactive bands sedimenting in fractions 8–12) is also shown. *p <0.05, **p <0.01 as assessed by the Student t or Mann-Whitney U tests