Fig. 5
From: Spinal cord injury induces astroglial conversion towards neuronal lineage
Astroglial transdifferentiation into GABAergic interneurons after SCI. Confocal micrographs showing parvalbumin and GAD 65/67 immunostaining in un-injured (a–d) and 6 weeks after FT lesion in Aldh1l1-EGFP mice (e–k). No eGFP/parvalbumin co-expression was observed in astrocytes of un-injured mice (a–d). Confocal mosaic micrograph showing the reference frames for fields of view displayed in F–I (e). At 6 weeks after FT triple eGFP/parvalbumin/GAD 65/67 expression was present within somata and primary cellular processes (arrow in f–i). A proportion of cells expressed double eGFP/GAD (arrowheads f–i) or eGFP/parvalbumin (j & k) proteins. Small insets in j and k are orthogonal projections of confocal z-stacks, which show eGFP co-localization with parvalbumin (confocal z-stack with XY, XZ, and YZ views). Scale bars(a–d & e & j) 50 μm and (f–i & k): 20 μm. (a–d) non-injured and (e–k) FT 6 weeks