Fig. 2

BACE1 is required for SEZ6 and SEZ6L shedding in primary neurons and mouse brain. a Schematic diagram of SEZ6 and SEZ6L domain structure and proposed proteolytic processing. b Detection of soluble SEZ6 and SEZ6L ectodomains (sSEZ6 and sSEZ6L) and full-length SEZ6 and SEZ6L in neuronal supernatant and lysate upon C3 treatment. c Detection of sSEZ6 and sSEZ6L and full-length SEZ6 and SEZ6L in BACE1 KO and WT brains. Brains were separated into soluble fraction (DEA) and membranes (membrane). Note that in this figure, a different molecular weight marker has been used compared to Fig. 1. The 148 kDa band corresponds to the band detected at 170 kDa in Fig. 1. The upper band in panel 2C (*) is due to unspecific signal. Densitometric quantitations of the Western blots are shown, (*; p < 0.05, **; p < 0.01, two-tailed Mann-Whitney test n = 6)