Fig. 6

SEZ6 is a substrate for γ-secretase. a HEK293T cells were stably transfected with empty vector (Empty) or SEZ6 expression construct with an N-terminal HA-tag and a C-terminal FLAG epitope tag. Cells were treated with C3 or DMSO as a control. sSEZ6 was detected in the cell supernatant and full-length SEZ6 in the lysate. Calnexin was used as a loading control. b Cells were treated with DMSO, C3 or the γ-secretase inhibitor DAPT. The C-terminal SEZ6 fragment was detected by immunoblot using an anti-FLAG-tag antibody