Fig. 3

a Anti-DMPO Western blotting showing α-synuclein radical formation. The reaction mixture containing α-synuclein (1.5 μM), cytochrome c (0.5 μM), and DMPO (50 mM) in the presence and absence of various concentrations of hydrogen peroxide (0, 25, 50 μM) was incubated at 37 °C for 1 h in chelexed phosphate buffer with 25 μM DTPA in a total volume of 200 μl. Samples were then used for electrophoresis and immuno-spin-trapping experiments using an anti-DMPO antibody. b Western blotting showing oligomerization of α-synuclein using the same reaction mixture and conditions as in A