Fig. 9
Sleep deprivation reduces the apoE3 arterial radial diffusion and clearance. a Schematic representation of the experimental design. Representative images at low (left) and high (right) magnification of the cortex (b) and hippocampus (c) in mice on normal sleep cycle. Representative images at low (left) and high (right) magnification of the cortex (d) and hippocampus (e) in sleep deprived mice. f of the distribution distance from the arteries in the cortex of control (sleep (S); clear column) and sleep deprived (SD; red column) mice. Fluorescently-tagged apoE3 was injected into cisterna magna of NG2dsRed reporter mice and perfusion fixed (PFA) at 15 min. The vasculature was outline by lectin (gray). ApoE, green; NG2-dsRed, red. Scale bars 100 μm (A-D, left), 50 μm (A-D, right). g Schematic diagram showing the intracortical injection site. h-i 125I-ApoE3 and 14C-inulin clearance from the frontal cortex in the sleep and sleep deprived states. Values are mean ± SEM. N = 5 mice per group