Fig. 4

p62 immunoreactive inclusion pathology is reduced in IL-6 expressing mice injected in the hippocampus with αSyn fibrils. Neonatal wild type mice were injected with rAAV-EV or rAAV-IL-6 and subsequently bilaterally injected in the hippocampus with αSyn fibrils at 2 months of age. Mice were analyzed 4 months post injection with αSyn fibrils in the hippocampus. Representative p62 staining is shown in different brain regions of rAAV-EV or rAAV-IL-6 mice following intra-hippocampal injection with αSyn fibrils (arrows, a). IL-6 expressing mice show considerably lower p62 levels than EV cohorts in response to hippocampal αSyn challenge. The number of hippocampal and cortical p62 inclusions were counted in IL-6 and EV cohorts injected with αSyn aggregates (b). *p < 0.05. n = 4–5 mice/cohort. Scale bar, 25 μm