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Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: The release and trans-synaptic transmission of Tau via exosomes

Fig. 2

Depolarization of neurons stimulates release of exosomes containing Tau. Cortical neurons were incubated in control medium or depolarizing medium containing 25 mM KCl for 3 h. a Cytotoxicity analyzed with LDH release assay. KCl treatment does not cause cell death. Error bars: SD; n = 3. Student t-test: n.s., not significant. b KCl treatment enhances exosomal Tau release. Neurons or exosomes isolated from conditioned medium of control neurons (lanes 1, 3) or depolarized neurons (lanes 2, 4) were lysed for western blot analysis. KCl treatment does not change Tau and Alix levels in neuronal cell lysates (compare lanes 1, 2), but increases both Tau and Alix in exosomes (compare lanes 3, 4). c, d Quantification of blots shown in B for Tau (upper panel) and the exosome marker Alix (bottom panel) in cell lysates (c) or exosomes (d). Shown are the ratios of proteins in cell lysates or exosomes (KCl-induced vs. control). Note that upon neuronal activation by KCl, Tau or Alix in exosomes increase by ~50% (d), whereas Tau or Alix in cell lysates does not change (c). Error bars: SD; n = 4. Student t-test: *p < 0.05; n.s., not significant

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