Skip to main content
Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: Glycine-alanine dipeptide repeat protein contributes to toxicity in a zebrafish model of C9orf72 associated neurodegeneration

Fig. 1

Generation of transgenic zebrafish model of C9ORF72 repeat expansion disease. a Schematic representation of Gal4 driver line zebrafish crossed to a UAS responder transgenic zebrafish to generate embryos that express a transgene under the control of the UAS. Schematic representation of the responder constructs used for the generation of transgenic zebrafish. b Genotyping by PCR of 1 dpf embryos. pCS2 + 2xGGGGGCC and pCS2 + 80XGGGGCC constructs were used as standards for GA2-GFP and GA80-GFP in lane 2 and 3. Positions of 2xGGGGCC and 80xGGGGCC repeats are indicated by arrows. c Semi-quantitative RT-PCR analyses for the wild-type, GA2-GFPa/b, GA80-GFPa/b and ggggcc80-GFP zebrafish. Note that all transgenic lines showed similar expression level at 4 dpf embryos. d Immunoblotting of wild-type, GA2-GFPa/b, GA80-GFPa/b and ggggcc80-GFP with antibodies as indicated with embryonic lysates of 4 dpf old embryos

Back to article page

Molecular Neurodegeneration

ISSN: 1750-1326