The SH-SY5Y cell line in Parkinson’s disease research: a systematic review

Table 2 Papers reporting the DAergic phenotype of the SH-SY5Y cell line and techniques used

DAergic phenotype	#articles	#differentiated
Not stated	432	70
Stated and not checked	392	50
PD model/DAergic properties/toxin	76	12
Stated and checked^a	48	22
Not stated but checked^a	7	3
Others	7	2
Technique	Single	Multiple
ICC	11 (4/7)	15 (6/9)
WB	10 (6/4)	23 (13/10)
qPCR	1 (1/0)	13 (7/6)
DA uptake/content	2 (2/0)	6 (3/3)
Not shown	4 (3/1)	0

The top part of the table indicates the number of papers that do specify or do not specify the DAergic phenotype of the SH-SY5Y cell line and whether or not the authors checked the phenotype. “Others” refers to articles mentioning other neuronal linages, including cholinergic, neuronal and noradrenergic phenotypes. Shown are the total number of articles (#articles) and the number of articles in which forced differentiation was employed (#differentiated). The bottom part of the table summarizes the techniques used in the publications that checked the phenotype (^a). Publications are divided into ‘single’ (i.e. papers that use only one method to check the DAergic phenotype) and ‘multiple’ (papers that use multiple complementary techniques). Between brackets: the number of studies that checked the DAergic phenotype in undifferentiated/differentiated cells. ICC immunocytochemistry, WB western blot, qPCR quantitative polymerase chain reaction, DA dopamine

ISSN: 1750-1326