Fig. 13

Lead decreases adult-born neuron differentiation in the DG of ApoE4-KI female mice. 8-week-old male and female ApoE3-KI and ApoE4-KI mice were exposed to 0.2% lead acetate for 12 weeks and then sacrificed. 100 mg/kg BrdU was administered 5 times in 1 day (every 2 h) 3 weeks prior to sacrifice (i.e., at 9 weeks into lead exposure). a Representative images of BrdU (red) and NeuN (green) immunostaining in the DG of control and lead-treated female ApoE3-KI and ApoE4-KI animals. Quantification of the percent of total BrdU+ cells that are BrdU + NeuN+ per DG in b females and c males, respectively. Quantification of the total number of BrdU + NeuN+ cells in d females and e males, respectively. Data are mean ± SEM with n = 4–5 per genotype/sex/treatment. Two-way ANOVA with Fisher’s LSD post-test: n.s., not significant; * p < 0.05; ** p < 0.01. Scale bar, 50 μm