Fig. 4

CAA analysis. APOE genotype differentially modulates the CAA load and its reduction during 10D5 mAb immunization. a Demonstration of CAA across APOE genotype spectrum. Representative microphotographs of Th-S stained coronal brain sections through the somatosensory cortex from untreated age-matched (Age control) mice, and mice receiving TY11-15 an isotype control IgG2a antibody or 10D5 anti-Aβ mAb. An arrow indicates a solitary CAA positive vessel in Age control APP/ε2 mouse. Arrowheads show long segments of CAA laden vessels in APP/ε4 mice and their reduction with 10D5 mAb treatment. b Mean (± SEM) of CAA load quantified in the brain cortex (n = 8-12/group). (b) p < 0.0001 (one-way analysis of variance); *p < 0.05, and ****p < 0.0001, TY11-15 control vs. 10D5 mAb treatment for matching APOE genotypes (Sidak’s post hoc test). ^## p < 0.01, APP/ε4 TY11-15 control vs. APP/ε3 TY11-15 control (Sidak’s post hoc test). Differences between Age control and TY11-15 groups were non-significant for matching APOE genotypes (Sidak’s post hoc test); not shown on the graph. Scale bar 100 μm (a)