Fig. 8

tPA induces chemokinesis on cultured OLs. a Rat primary cultures of OPCs were seeded on the upper compartment of Boyden chambers and treated in indicated conditions for 24 h. Photomicrographs show representative fields of the lower compartment after Olig2 immunostaining (green; representative image, n = 9, three independent cultures). b Oligodendrocyte chemokinesis was estimated by counting cells in the lower compartment in control conditions or in the presence of 0.2 μg/mL FGF2, 0.1-10 μg/mL tPA or tPA vehicle. (mean ± SEM; n = 9, three independent cultures; *p < 0.05 and **p < 0.01). c OL proliferation (percentage) during 24 h was estimated by measuring mitochondrial activity with WST-1 test after treatment or not. Control is the basal OL proliferation during 24 h without treatment (mean + SEM; n = 15, five independent cultures). OPC: oligodendrocyte precursor cell; ns: not significant. Scale bars: 100 μm