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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: Spatiotemporal progression of ubiquitin-proteasome system inhibition after status epilepticus suggests protective adaptation against hippocampal injury

Fig. 3

UPS impairment in the contralateral hippocampus after status epilepticus. a. Representative photomicrographs (5x lens) showing subfield-specific increased GFP-reporter accumulation in the contralateral hippocampus of transgenic UbG76V-GFP mice subjected to intra-amygdala KA-induced status epilepticus. Note, similar GFP staining pattern as observed in the ipsilateral hippocampus. Scale bar, 500 μm. b. Representative Western (n = 1 per lane) and graphs showing increased GFP levels in the contralateral hippocampal subfields DG, CA1 and CA3 after status epilepticus (mean ± sem, *p <0.05 by one-way ANOVA with Fisher’s post hoc test; n = 4 per group). c. Representative Western (n = 1 per lane) and graph showing increased polyubiquitination levels (FK2 antibody) in the contralateral hippocampus after intra-amygdala KA induced-status epilepticus (mean ± sem, *p <0.05 by one-way ANOVA with Fisher’s post hoc test; n = 4 per group). d. Graphs showing no significant changes in GFP mRNA levels 4 h and 8 h after status epilepticus for the contralateral hippocampal subfields DG, CA1 and CA3 (mean ± sem, by one-way ANOVA with Fisher’s post hoc test; n = 4 per group). ns = non-significant

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