Fig. 4
Recovery of Aβ-induced ROS and Ca2+ by HDAC6 inhibitor is mediated by acetylation of Prx1. a Representative immunoblot showing expression of Prx1-WT or K197Q or K197R-Flag in HT22 cells. Immunoprecipitated exogenous Prx1-Flag showed differential detecting pattern of acetyl-Prx1 among wild-type and mutants Prx1. Exogenous Prx1-Flag was probed by anti-Flag antibody. Beta-actin is loading control. N.C.: Negative Control. b, c Prx1-WT or K197Q or K197R-Flag transfected HT22 cells were treated with Aβ (2 μM, 24 h) followed by incubated with TBA (0.5 μM, 3 h). Quantitative graph of DCFDA assay for ROS measuring (b) and Fluo-4 assay for Ca2+ measuring (c) showed that acetylation of Prx1 regulates ROS and Ca2+ level in the presence of Aβ, respectively. Data are presented as mean ± SEM. (b: n = 10, c: n = 7, independent experiments) *P < 0.05, **P < 0.01, ***P < 0.001, n.s.: non-significant (two-way ANOVA, Bonferroni post-hoc test)