Fig. 5

Peptide profile of robust Parkin responders encompassing a Di-Gly peptide. Di-Gly peptides were detected for 5 of the Parkin substrates identified: Cisd2, EIF2B-beta, Pdh, Porin and SesB. Moreover, Cbp53E and NinaE, whose ubiquitination robustly decreases in a Parkin E3 ubiquitin ligase activity dependent manner, also provided a di-Gly peptide. Three ubiquitination sites were also detected in Parkin itself. The graph represents the peptide intensity of all the unique peptides that were assigned to the indicated protein in each condition (grey dots). Di-Gly peptides are shown in blue and the average peptide intensity in red