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Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: Separation of photoreceptor cell compartments in mouse retina for protein analysis

Fig. 2

Light-induced movement of GNAT1 and ARR1 in rod photoreceptors. a Frozen retinal sections prepared from dark-adapted or light-exposed mice incubated with GNAT1 or ARR1 antibodies (green). The location of the rod outer segment is visualized with an antibody against rhodopsin (red), shown at the right of each panel. The diagram of the rod cell depicts the position of each rod compartment on the retinal section. Scale bar = 20 μm. b Representative immunoblots of ROS collected by filter paper peeling of retinas obtained from dark-adapted (D) or light exposed (L) mice. The -ROS fraction is the ROS-depleted tissue. c Quantified signals from light exposed (n = 6) and dark adapted (n = 5) +ROS and -ROS samples plotted as mean ± SD. There was a statistically significant difference between +ROS (D)/+ROS (L) (p < 0.0003) and -ROS (D)/-ROS (L) respectively for GNAT1 (p < 0.0001) and ARR1 (p < 0.0001) using unpaired t-test. There was no statistically significant difference between +ROS (D)/+ROS (L) and -ROS (D)/+ROS (L) for RGS9 (p = 0.8)

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