Fig. 5

Change in retinal chemokine expression and macrophage infiltration in retinas following intravitreal injection of IL-1β protein. a Injection of IL-1β protein increased the expression of Ccl2, Cxcl1 and Cxcl10 in retinas over a time-course of 6, 12, and 24 h post-injection, compared to PBS-injected controls (P < 0.05). b There was a significant increase in the number of IBA1+ cells throughout retinal sections in the IL-1β group at 24 h post-injection, compared to the PBS control group (P < 0.05). c-f Representative images of IBA1-immunolabelled retinal sections show increased clusters of IBA1+ macrophages amongst the GCL and optic nerve head of the IL-1β-injected group (e-f; arrows) compared to PBS controls (c-d). g-h Representative images show positive expression of PCR products for Il-1r1 and Il-rap in control rat retinas. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer. N = 3-6 per group. Asterisks denote a significant change, where P < 0.05. Scale bars equate to 50 μm