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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: Prevention of C5aR1 signaling delays microglial inflammatory polarization, favors clearance pathways and suppresses cognitive loss

Fig. 3

Glia clustering around plaques are CX3CR1+CCR2−. Brain sections of (a) WT, (b) Arctic, (c) C5aR1KO, and (d) Arctic/C5aR1KO mice (all CX3CR1+/GFPCCR2+/RFP) at 2, 5, 7, and 10 months were fluorescently labelled in blue with anti-Aß antibody (1536). Staining was performed at separate times for cohort one (a,b) and cohort 2 (c,d). Representative pictures of cohorts one and two were taken at a close but slightly different bregma levels. Inset shows CCR2+/RFP macrophages. Scale bar:50 um (e) Percoll purified brain cells from WT, Arctic, C5aR1KO and Arctic/C5aR1KO mice (all CX3CR1+/GFPCCR2+/RFP; n = 4–12 per genotype, per age) were FACS-sorted as GFP+RFP+ (e,g) and GFP+RFP− cells (f,h) and quantified as a percentage of total GFP+ cells (e,f) and total cell count (g,h)

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Molecular Neurodegeneration

ISSN: 1750-1326