Fig. 1

Proteomic screens identify increases in cytosolic HK1 in the brains of DJ-1 knockout rodents. a-b Volcano plots displaying abundance of 994 proteins in mitochondria-enriched fractions (a) and 957 proteins in cytosol-enriched fractions (b) of 6-month-old male rat brains (n = 7 WT, n = 7 DJ-1 knockout). Significantly altered proteins (Benjamini Hochberg (BH) adjusted Welch’s t-test p < 0.05) are labeled with their official gene names. The DJ-1 points were omitted from the graph for clarity but are located at (−2.237, 10.02) in (a) and (−2.95, 8.99) in (b). (c) Volcano plot of 742 proteins quantified in the cytosol-enriched fraction of 14–15-month-old mouse brains (n = 9 WT (5 M, 4 F), n = 5 DJ-1 knockout (3 M, 2 F)). NEFL, NEFM, and NEFH are labeled but did not reach statistical significance (BH adjusted Welch’s t-test p < 0.05). DJ-1 was omitted from the graph but is located at (−1.99, 3.97). (d-f) Western blots showing levels of HK1 protein in the cytosol-enriched fraction of rat brains (d, 6-month-old males, n = 7 WT, 7 DJ-1 knockout),1-month-old mouse brains (e, n = 8 WT (4 M, 4 F), n = 8 DJ-1 knockout (4 M, 4 F)), 4-month-old mouse brains (f, n = 9 WT (4 M, 5 F), n = 8 DJ-1 knockout (5 M, 3 F)), and 14–15-month-old mouse brains (g, n = 9 WT (5 M, 4 F), n = 5 DJ-1 knockout (3 M, 2 F)). β-Actin was used as a loading control. Graphs display the mean, standard error of the mean (SEM), and * indicates p < 0.05 (Welch’s t-test)