Table 1 Combined Jun/Ddit3 deficiency is more protective after axonal injury than Jun or Ddit3 deficiency alone. (A) RGC per mm2 and (B) Percent survival was calculated for WT, Ddit3 deficient, Jun deficient, and combined Jun/Ddit3 deficient retinas at 14, 35, 60, and 120 days after ONC. Percent survival was defined as the mean number of RGCs after ONC divided by the mean number of RGCs after SHAM. Values are displayed plus/minus SEM. (C) One-way ANOVA followed by the Tukey’s post hoc test for multiple comparisons was used to analyze RGC cell counts at 14, 35, 60, and 120 days after ONC
From: Together JUN and DDIT3 (CHOP) control retinal ganglion cell death after axonal injury
A. RGC per mm2 | ||||
|  | WT | Ddit3 −/− | Jun −/− | Jun −/− Ddit3 −/− |
14 Day ONC | 976.4 ± 59.1 | 1625.4 ± 62.8 | 2954.5 ± 128.5 | 3053.9 ± 48.6 |
14 Day SHAM | 3306.8 ± 47.3 | 3191 ± 25.0 | 3178.5 ± 73.9 | 3060.6 ± 42.5 |
35 Day ONC | 681.4 ± 92.5 | 1484.8 ± 68.7 | 2340.5 ± 98.8 | 2729.8 ± 83.9 |
35 Day SHAM | 3149.4 ± 51.7 | 3125.8 ± 30.6 | 3103.5 ± 51.7 | 3085.4 ± 48.0 |
60 Day ONC | 427.2 ± 79.7 | 1041.4 ± 14.0 | 1635.9 ± 79.7 | 2577.4 ± 100.4 |
60 Day SHAM | 3166.7 ± 42.4 | 3122.9 ± 61.1 | 3006.3 ± 42.4 | 3108.2 ± 19.9 |
120 Day ONC | 219.0 ± 8.3 | 797.0 ± 40.1 | 1498.0 ± 88.3 | 2328.0 ± 179.4 |
120 Day SHAM | 3178.0 ± 30.1 | 3116.0 ± 52.4 | 3101.1 ± 38.9 | 3086.0 ± 61.1 |
B. % Survival (ONC/SHAM) | ||||
|  | WT | Ddit3 −/− | Jun −/− | Jun −/− Ddit3 −/− |
14 Day | 29.5 ± 1.8 | 50.9 ± 0.6 | 93.0 ± 1.0 | 99.8 ± 0.3 |
35 Day | 21.6 ± 2.0 | 47.5 ± 2.2 | 75.4 ± 0.4 | 88.5 ± 1.3 |
60 Day | 13.5 ± 4.0 | 33.3 ± 3.2 | 54.4 ± 2.6 | 82.9 ± 2.8 |
120 Day | 6.9 ± 1.6 | 25.3 ± 2.7 | 48.3 ± 3.2 | 75.4 ± 5.8 |
C. One-way ANOVA followed by the Tukey’s post hoc test | ||||
               14 Days after ONC | ||||
|  | WT | Ddit3 −/− | Jun −/− | |
Ddit3 −/− | <0.0001 | – | – | |
Jun −/− | <0.0001 | <0.0001 | – | |
Jun −/− Ddit3 −/− | <0.0001 | <0.0001 | 0.2107 | |
               35 Days after ONC | ||||
|  | WT | Ddit3 −/− | Jun −/− | |
Ddit3 −/− | <0.0001 | – | – | |
Jun −/− | <0.0001 | <0.0001 | – | |
Jun −/− Ddit3 −/− | <0.0001 | <0.0001 | 0.0024 | |
               60 Days after ONC | ||||
|  | WT | Ddit3 −/− | Jun −/− | |
Ddit3 −/− | <0.0001 | – | – | |
Jun −/− | <0.0001 | <0.0001 | – | |
Jun −/− Ddit3 −/− | <0.0001 | <0.0001 | <0.0001 | |
               120 Days after ONC | ||||
|  | WT | Ddit3 −/− | Jun −/− | |
Ddit3 −/− | <0.0001 | – | – | |
Jun −/− | <0.0001 | <0.0001 | – | |
Jun −/− Ddit3 −/− | <0.0001 | <0.0001 | <0.0001 | |