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Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: The CNS in inbred transgenic models of 4-repeat Tauopathy develops consistent tau seeding capacity yet focal and diverse patterns of protein deposition

Fig. 1

Total human Tau and total phosphorylated Tau in 3 congenic strains of TgTauP301L mice. a schematic of the cosmid transgene and derivation of a founder Tg line (open arrow). A Toronto breeding stock (T, indicated by dashed square) was used to generate three inbred derivatives. In panels b and c, brain homogenates of Tg mice from all 3 congenic strains and controls were analyzed for total Tau and total human Tau expression levels using western blot. b A representative blot showing total Tau expression in Tg mice of all congenic strains and control animals. Graph represents relative expression levels of total Tau normalized to actin loading control and then compared to control non-Tg mice n = 2 per Tg sample per inbred stain with values for non-Tg sample pooled from the three strains and assigned the value “1.0”. Antibody is DA9:1/500). TgTauP301L mice have ~1.7X Tau expression level (1.77, 1.61, 1.69 for C57BL/6Tac, 129/SvEvTac and FVB/NJ respectively) compared to controls. c A representative blot showing total human Tau expression level in Tg mice and controls. Graph represents total human Tau expression levels in transgenic mice of all 3 congenic strains (n = 3 each) normalized to C57BL/6Tac (100%; antibody CP27, 1/500). d Total human Tau distribution (CP27) in brain of Tg mice (280–324 days old) from C57BL/6Tac (ii), 129/SvEvTac (iv) and FVB/NJ (vi) backgrounds, with corresponding non-Tg animals shown in panels i, ii and iii

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