Fig. 12

Insoluble Tau species in the brains of aged TgTau^P301L mice. Fractionated brains comprising supernatant pellet 1 (SUP1), supernatant pellet 3 (SUP3), and pellet 3 (P3) of aged TgTau^(P301L) mice were analyzed by western blot analysis. One example of Class I, II and IV is shown for each genetic background. a C57BL/6Tac mice at ages 587, 732, and 530 days left to right (b), 129/SvEvTac at ages 662, 592, and 466 days left to right, and (c) FVB/NJ mice at ages 646, 658, and 639 days left to right. For all samples, 10 μg of total protein was loaded on the gel. Antibody: CP13 (1/500; phosphoserine 202). SUP = Supernatant, and P = pellet fractions. d P3/S3 ratios of animals presented in A-C. Ratios were pooled across strain backgrounds. Class I vs. II p = 0.020; I vs IV, p = 0.08; II vs, IV, p = 0.003. e S1P fraction of animals with classes I, II and IV pathology to show the presence of oligomeric species in the soluble extract, antibody: CP27 (1/500)