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Fig. 13 | Molecular Neurodegeneration

Fig. 13

From: The CNS in inbred transgenic models of 4-repeat Tauopathy develops consistent tau seeding capacity yet focal and diverse patterns of protein deposition

Fig. 13

Trypsin-resistance of sarkosyl-insoluble Tau fractions. a A schematic of antibody epitope is presented. b 10 μg of P3 fractions from animals of classes I, II and 15 μg of P3 for animals of IV were subjected to trypsin digestion (1/100 for enzyme/protein ratio) and analyzed by western blotting. The banding patterns in samples are represented before and after trypsin-digestion. The samples are organized by age in an increasing order. Animals from class I, left to right C57BL/6 J, FVB/NJ, two C57BL/6 J and FVB/NJ. Animals from class II, left to right C57BL/6 J, two 129/SvEvTac, FVB/NJ and C57BL/6 J and 129SvEv/Tac and two FVB/NJ and C57BL/6 J animals from class IV. The exposure time for different paired samples electrophoresed and transferred from the same gel was adjusted to obtain similar signal intensities for the predominant immunoreactive species. ET3 anti-Tau (4R specific, residues 273–288) was used to detect Tau fragments at 1/250 dilution

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Molecular Neurodegeneration

ISSN: 1750-1326