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Fig. 15 | Molecular Neurodegeneration

Fig. 15

From: The CNS in inbred transgenic models of 4-repeat Tauopathy develops consistent tau seeding capacity yet focal and diverse patterns of protein deposition

Fig. 15

Assessment of Tau strains in a cell-based seeding assay. Upper panel: Mouse brains have been cut saggitaly with the right hemispheres fixed in formalin for further processing and embedding in paraffin and for use in immunohistochemistry. Left hemispheres were cut transversally according to the diagram (dashed line). Each rostral or caudal portion was then homogenized and used as a seed on cell cultures as described. Lower panels represent the fluorescent micrographs obtained from seeding assays with rostral (red border) or caudally-derived (blue border) derived homogenates. When transduced into Clone 1 cells, which express Tau RD-YFP but lack aggregates, all homogenates seed morphologically indistinguishable Tau inclusions, which feature tangles of filamentous Tau (panels a-l). These inclusions are morphologically distinct from those seeded by Clone 9 (nuclear speckles) and Clone 10 (ordered inclusion) lysates (see Additional file 10: Figure S21)

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