Fig. 1

Increased soluble and insoluble tau phosphorylation in TREM2 deficient mice. a-d Microdissected cortices and hippocampi from hTau (Trem2 ^+/+) and hTau;Trem2 ^−/− mice were analyzed using western blot with antibodies against AT8, AT180, PHF-1 phospho-epitopes and Tau5 (total tau). b Quantification of cortex western blot data revealed highly significant increases in the ratio of phosphorylated AT8, AT180, and PHF-1 to total tau but importantly, no increases were detected in total tau (Tau5). d Quantification of hippocampus western blot data reveals significant hyperphosphorylation at the AT8 epitope. e Sarkosyl extractions were performed on cortical tissue lysates from hTau (Trem2 ^+/+) and hTau;Trem2 ^−/− mice and protein levels analyzed using western blot. f Quantification of western blot optical densities revealed significant increases in AT180 and highly significant increases in PHF-1 and Tau5 (total tau). All experiments used n = 4–6 (equal males and females) mice per group unless otherwise noted. At least two independent experiments were performed for each analysis. Error bars represent SEM. *, P < 0.05, **, P < 0.01, ***, P < 0.001