Fig. 7
From: Modulating the catalytic activity of AMPK has neuroprotective effects against α-synuclein toxicity
Effects of AMPKα and α-syn on mitochondria in cortical neurons. a Average mitoDsRed fluorescence intensity measured by flow cytometry in individual neuronal cultures. Note the significant increase in the mitoDsRed fluorescence intensity in neurons co-expressing AMPKα2 and α-syn, as compared to the control. b Real-time PCR quantification of mtDNA (16S) versus cellular gDNA (Hk2). Note that α-syn overexpression results in overall decline in the relative amount of mtDNA per cell. In cortical neurons overexpressing AMPK T172Dα1, the amount of mtDNA is significantly lower, regardless of α-syn being overexpressed. Statistical analysis: two-way factorial ANOVA with Newman-Keuls post hoc test; for mitochondrial mass, n = 10–12 per condition; for mtDNA, n = 4 per condition, except for α1 + α-syn (n = 3); *P < 0.05