Fig. 13
From: A validated antibody panel for the characterization of tau post-translational modifications
Immunofluorescence staining reveals a large number of tau phosphorylation events between amino acids S238 and S422 in hiPSC-derived neurons. All phospho-tau antibodies in the panel showed staining in hiPSC-derived neuronal cultures. While some antibodies stained both the cytoplasm and the cell body (b, e, f, g), pS238 signal was exclusively in the cell body (a). Antibodies against pS396 (c), pS400 (d), and pS422 (h) on the other hand preferentially stained neurites. Green: antibody staining, blue: Hoechst staining. Scale bars represent 100 μm