Fig. 4
From: A validated antibody panel for the characterization of tau post-translational modifications
Peptide arrays validate the specificity of phospho-tau antibodies covering aa 235–422. a pS235, b pS238, c pS356, d pS396, e pS400, f pS404, g pS409, h pS416 and i pS422 antibodies demonstrated specific detection of the respective modifications on a peptide array. While all antibodies are specific, the detection of pS235 (a), pS396 (d) and pS400 (e) can be inhibited by additional phosphorylation events in the surrounding peptide sequence. The sequence and modification profile for peptides immobilized in each spot are shown below the blots. The modification targeted by the antibody is shown in red, off-target modifications are shown in bold black