Fig. 7

Switching-off the expression of mutant Tau. 2μM DOX was applied to the culture media of the slices to switch-off the expression of the exogenous human Tau constructs from DIV15 to DIV30. Bioluminescence was done after the DOX application on the slice cultures to make sure that there is no further expression of the anti-aggregant Tau^RDΔKPP during the subsequent culturing period. Results show the mean ±SEM of 10 animals, 4-6 slices per animal. Data was analyzed by Student's t test. *p<0.05 and **p<0.01 compared to switch-ON and switch-OFF conditions. a Graph representing the number of BrdU positive proliferating cells in the control and anti-aggregant Tau^RDΔKPP OHSCs before DOX application (switch-ON) and after (switch-OFF). There was a reduction of 32% in CA1, 27% in CA3 region and 33% in DG region in the number of BrdU positive cells in the DOX treated anti-aggregant Tau^RDΔKPP slices compared to the untreated anti-aggregant Tau^RDΔKPP slices (see pink open vs. hatched bars). This reduction parallels the decrease in the number of neurons in DOX-treated anti-aggregant Tau^RDΔKPP groups (see b). b Graph representing the number of NeuN positive neurons per counting frame, in the control and anti-aggregant Tau^RDΔKPP OHSCs before DOX application (switch-ON) and after (switch-OFF). There was a reduction of 21% in CA1, 22% in CA3 and 37% in DG region of the hippocampus, in the number of NeuN positive mature neurons in the DOX treated anti-aggregant Tau^RDΔKPP slices compared to the untreated anti-aggregant Tau^RDΔKPP slices (see pink open vs. hatched bars). Data was analyzed by Student's t test. *p<0.05, ** p<0.01 and ****p<0.0001 compared to controls