Fig. 4

Protein quantitation overview and comparative pathway enrichment analysis of ADLP model mice. a Hierarchical clustering of differentially expressed proteins (DEPs) across different transgenic types and ages (ANOVA FDR < 0.05). Protein expression profiles were largely clustered into three patterns; The decreasing pattern (Cluster 2) and the increasing pattern (Cluster 3) of the mouse models with human APP (ADLP ^APP/PS1, ADLP ^APT) are prominent. The right panel shows the Z-normalized protein abundance according to the mouse samples as profile plots. b Canonical pathway enrichment for DEPs. The categories of nervous system signaling and immune response were overrepresented (~50%) and are highlighted. The significant pathways (Fisher’s exact test p-value <0.05) were deduced using Ingenuity Pathway Analysis (IPA) and their predictive activation/inhibition status is represented as the Z-score. *APP/PS1 = ADLP^APP/PS1, Tau = ADLP^Tau, APT = ADLP^APT mice