Fig. 6
From: Piperine ameliorates SCA17 neuropathology by reducing ER stress
Piperine treatment increased ATF6 expression in TBP-105Q KI mice. a Western blotting analysis of full length (ATF6(fl)) and N-terminal ATF6 (ATF6(N)) in the cortex of WT and TBP-105Q KI (TBP KI) mice at 6 months of age. Five mice per group were analyzed. b Quantification of the ratios of ATF6(fl) or ATF6(N) to actin on western blots shown in Fig. 6a (n = 5, * P < 0.05). c Western blotting analysis of full length and N-terminal ATF6 in the cortex of TBP-105Q KI mice treated with either saline (TBP KI + saline) or piperine (TBP KI + Pip) for 8 weeks. Four mice per group were used for analysis. d Quantification of the ratios of ATF6(fl) or ATF6(N) to actin on western blots shown in Fig. 6c (n = 4, ** P < 0.01). Results are presented as means ± SEM. e Immunohistochemistry staining of ATF6 in the brain of TBP-105Q KI mice treated with either saline or piperine for 8 weeks (Scale bars: upper, 50 μm; lower, 20 μm)